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Rat MCL cells were loaded with Fura-2AM, a calcium-binding dye, and mechanically indented using a micropipette tip.
The adherent monolayer cells were scratched by using a micropipette tip and washed twice with PBS to remove suspended cells.
A scratch wound was created on the cell surface using a micropipette tip.
After a 6 hs long serum starvation, a wound was made by using a micropipette tip to scrape off the cells.
A wound was generated using a micropipette tip before washing to remove cell debris.
After 48 h, a distinct area of the monolayer culture was wounded using a micropipette tip.
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Briefly, 1 × 10 HLECs were plated in 6 well plates and synchronized by serum-free ECM medium for 8 h, then used a micropipette tip to create a 2 mm-wide linear gap.
This mixture was then microinjected using a micropipette (∼1-μM tip diameter).
Because neonatal mouse pups are too small to safely dose by intragastric gavage, the decaBDE emulsion was administered using a micropipette with 200-μL tips at a concentration of 5 μL/g body weight.
The supernatant is loaded using a micropipette onto a gel loading tip column with fiber glass as a frit containing the appropriate amount of fresh immobilized metal affinity chromatography (IMAC) resin (usually ∼60 μl; see above).
and atomic-force microscopy (AFM) tips [ 34 – 37 ]; electroporation using a micropipette filled with nucleic acids and an electric field [ 38 ]; and phototransfection using a multi-photon laser [ 20 ]. micro-injections using a very small glass needle, nano-needle, femtosyringe.
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