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Integrated with the three-dimensional (3-D) nanofibrous skeletal structure of native extracellular matrix, electrospun fibers with gradients in amino groups were generated in the current study through an aminolysis process by using a microinfusion pump.
Pressure injections were performed using a microinfusion pump as described above; injection volumes were 100 nl (Exp. 4).
Drugs were delivered using a microinfusion pump (Harvard Apparatus, Holliston, MA) connected to a 33-gauge microsyringe injector, via indwelling guide cannulae.
For collection of dialysates, the probes (Microbiotech) were perfused at 2 μl/min with Ringer's solution by using a microinfusion pump (11 Plus; Harvard Apparatus GmbH, Hugstetten, Germany).
Each solution was continuously infused for 120 min into the tail vein at a rate of 0.015 ml min−1 by using a microinfusion pump (Compact Syringe Pump; Harvard Apparatus).
The probe was perfused continuously overnight with sterile artificial cerebrospinal fluid at 0.3 μL/min using a microinfusion pump to equilibrate extracellular metabolites and to allow the animal to acclimatise to the liquid swivel counterbalance arm system.
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Bacterial suspensions or PBS were infused at a rate of 40 nl/min over 10 min into the lumen of superficial proximal tubules using a micromanipulator and a microinfusion pump.
Animals were injected with AMPA (15 mM) in a volume of 0.5 μL of 0.9% saline using a Hamilton syringe in a microinfusion pump (KD Scientific Co., USA) at a rate of 0.5 μL/min (n = 5, coordinates: AP = +0.7 mm and L = ±2.7 mm (left n = 2, right n = 3), relative to bregma; DV = −5.5 mm from dura) [ 36].
A microinfusion pump (KD scientific, Holliston, MA) was used to maintain the speed of delivery at 0.5 μL/min.
Polyethylene tubing (PE50) was connected to a microinfusion pump (WPI Inc, Sarasota, FL, USA) as well as to the exposed cannula.
The infusion cannulae were attached with PE50 to Hamilton syringes (Hamilton, Reno, NV) controlled by a microinfusion pump.
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