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Blood samples were collected once per 2-week trapping session from the suborbital venous plexus by using a microhematocrit capillary tube (length 75 mm, diameter 1.15 mm).
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The PCV was measured using a microhematocrit centrifuge and a Hawksley reader (Hawksley & Sons Ltd ,Lancing, UK).
The PCVs were measured using a microhematocrit tube and microcentrifuge (Hawksley, Lancing, UK).
Blood was collected using Heparinized Microhematocrit Capillary Tubes (Thermo Fisher Scientific) and centrifuged at 15,000× g to obtain plasma.
Injections were performed using a glass capillary attached to a microsyringe (Hamilton, Reno, NV) at a 0.7 mm depth.
The injection was performed using a glass capillary attached to a syringe (Hamilton, Reno, NV) and a micropump (Ultramicropump III, World Precision Instruments, Sarasota, FL).
PCR amplification products were resolved using a QIAxcel capillary system.
For cationic metabolites, capillary electrophoreses were performed using a fused silica capillary.
Glass capillaries (5 µl) were modified using a capillary puller (PN-30, Narishige, Tokyo, Japan).
Purity of the different cultures was checked using a capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) analysis.
The rheological behaviour was determined using a capillary rheometer.
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