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The suspension containing yeast cells, collagen and media was printed (50 nL per spot) on the functionalized PSMA-coated glass slides using a microarray spotter (Omnigrid Micro, Digilab Inc., Holliston, MA).
Probes were spotted onto washed glass slides using a microarray spotter, Omnigrid Accent (GeneMachines, San Carlos, California), under a humidity of 50-55% and at a temperature of 22-25°C 22-25°C
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To measure cytokine levels, anti-human IFNγ capture antibody (Thermo Fisher, Waltham, MA), rabbit anti-SEA antibody (negative control; Toxin Technology), and Alexa647-labeled streptavidin (positive control; Invitrogen) were arrayed onto PATH Protein Microarray slides (Gentel Bioscience, Madison, WI) using an inkjet microarray spotter (ArrayJet, Roslin, Scotland, UK).
In this work, an instrument is used to acquire spatial maps of the resonant reflected wavelength in order to produce images of bound biomolecule patterns as applied by a microarray spotter.
The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences).
These probes were printed onto aldehyde slides (Genetix) using a Stanford type microarray spotter in house [50], [51].
DNA microarrays were produced using a contact spotter (built according to the MGuide, Stanford).
We have found during optimization (data not shown) of protein microarray production that proteins concentrations of up to 0.5 mg/mL are well suited for spotting using a contact spotter.
The PCR products were then arrayed in triplicate on CodeLink Activated Slides (Amersham Biosciences) using a GMS417 spotter (MWG Biotech).
A set of 109 immune, endocrine and inflammation-associated genes was selected for triplicate spotting onto Corning GAPS II slides using a VIRTEK Chip Maker Pro spotter (BioRad, Mississauga, Canada).
Microarrays were printed using a Lucidea Array Spotter (Amersham) at the Array Core facility at UC Davis (since closed down).
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