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Moreover the intensity of gene expression, assessed using a microarray reader, was considerably higher for AAV8 in all structures analyzed.
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Finally, fluorescence images were captured using a microarray scanner (Axon).
Genomic-wide expression profiling was performed in NC- and miR-139-5p-transfected miR-139-5p-transfected miR-139-5p-transfected miR-139-5p-transfected
Fluorescent signals were detected using a DNA microarray reader (BIOCCD Image Reader, PE Applied Biosystems) with excitation 470/30 and emission 510/20 filters for EGFP and ZsGreen.
Raw intensity data was generated using an iScan microarray reader (Illumina).
using a plate reader.
Luminescence was recorded using a plate reader.
using a plate reader (Thermo Fisher Scientific Inc., MA, United States).
Lastly, absorbance was measured at 490 nm using a microplate reader (Bio-Tek Synereader micreadere reader).
Absorbance at 540 nm was measured using a microplate reader (iMark microplate reader; Bio-Rad).
Luminescence was measured using a Victor3 reader (Perkin Elmer).
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