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Spot intensities are determined using a microarray image analysis software (GenePix).
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Fluorescence was then quantified using a microarray scanner PerkinElmer and images were analyzed using Scanarray Express software.
Images of the arrays were acquired using a microarray scanner G2565BA (Agilent technologies) and image analysis was performed using Feature Extraction software version 9.5 (Agilent Technologies).
Images of the arrays were acquired using a microarray scanner G2505B (Agilent technologies) and image analysis was performed using feature extraction software version 9.5 (Agilent Technologies,).
After hybridization and washing, the slide was scanned using a microarray scanner, generating two color images showing spot intensities for the gene of interest and for 18S ribosomal RNA (Fig. 1A,1B,1C).
Finally, fluorescence images were captured using a microarray scanner (Axon).
Images were acquired using a Microarray Scanner G2505B (Agilent Technologies).
Numeric data was extracted from the two-channel hybridization images using the microarray image analysis software, BlueFuse (BlueGnome Ltd ,Cambridge).
Fluorescence intensities were recorded, and data processing was performed by using the Microarray Image Analysis software (Phadia).
The resulting tiff images (10 μm/pixel) were analyzed using dedicated microarray image analysis software (GenePix Pro 6.0 software, Molecular Device, Sunnyvale, CA, USA) that located each spot automatically (with manual control) and quantified its fluorescence intensity.
After hybridization, arrays were scanned on a fluorescent microarray scanner and images analyzed using microarray image analysis software.
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