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Sera were also tested for the presence of virus neutralizing antibodies specific for the two influenza viruses using a micro virus neutralization (VN) assay with the viruses that were produced by reverse genetics as described above [40].
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The samples were screened for neutralizing antibody against the equine WNV strain, Morocco 96 111 (3 ), by using a micro virus-neutralization test in 96-well plates and an adaptation of a described method (5 ).
Neutralizing antibody titers against WNV (strain Spain/2007 GE-1B/b) and Usutu virus (USUV) (SAAR1776) were determined by using a micro virus-neutralization test as described previously (5 ).
Neutralizing antibody titers for WNV (strain Eg101) were determined by using a micro-virus neutralization test as described (6 ).
Peptide concentration was determined using a micro bicinchoninic acid assay (Micro BCA Assay; Thermo Scientific).
Supernatants were collected on days 3, 7, 10 and 14 post-infection and virus infectivity was measured by monitoring the production of viral RT activity by using a micro-RT assay.
The robot's blinking eyes and mouth are controlled using a micro-controller and a PC.
Hybridization was performed using a micro-circulation pump (Atactic Technologies).
All of samples were scanned using a micro-CT scanner (Inveon Micro-CT, Siemens, Germany).
Costs were estimated using a micro-costing approach.
The protein concentration was estimated using a micro-BCA assay.
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