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The optical density was measured at 540 nm using a micro plate reader.
The resulting purple formazan crystals were solubilized in 100 µL of DMSO and the absorbance was measured at 570 nm using a micro plate reader (EMR500-Labomed).
The medium was removed and 100 μL DMSO was added and the absorbance measured using a micro plate reader at 540 nm followed by the calculation of percentage viability 0.1% (v/v) DMSO in medium was used as negative control.
The medium with MTT was then flicked off and the formed formazan crystals were solubilized in 100 μl DMSO and absorbance was measured at 570 nm using a micro plate reader.
The extent of MTT reduction to formazan within cells was calculated by measuring the absorbance at 570 nm, using a micro plate reader (Spectra Max plus, Molecular Devices, CA, USA).
Cell activity after 72 h of further culture was measured by 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyl tetrazolium bromide assay (MTT) with optical density at 490 nm (OD490 nm) using a micro plate reader (EL×800, BioTek, Winooski, VT, USA) (n = 5).
Similar(46)
The emitted fluorescence of samples at 450 nm was measured using a micro-plate reader (UVM 340, Biochrom, Cambridge, UK).
The absorbance was measured at 450 nm using a micro-plate reader (Bio-Rad Model 680, Richmond, CA, USA).
After incubating for 20 min, the absorbance was measured using a micro-plate reader (Thermo Fisher Scientific Co., Waltham, MA) at 570 nm.
The supernatant (80 μL) and 20 μL of L-DOPA (2 mg/mL) were placed in a 96-well plate, and the absorbance at 492 nm was read for 30 min at 37°C using a micro-plate reader.
The optical density at 548 nm was measured using a micro-plate reader.
More suggestions(15)
using a micro coil
using a micro reactor
using a micro end
using a micro dose
using a micro electro
using a micro control
using a micro pestle
using a micro pipette
using a micro rotofor
using a standard plate
using a micro virus
using a micro caliper
using a hot plate
using a fluorescent plate
using a parallel plate
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