Your English writing platform
Free sign upSuggestions(1)
Exact(2)
Immediately after FFE fractionation, the pH in each well of the microtiter plate was measured using a micro pH electrode.
Fluorescence was calibrated in the presence of nigericin (pHin = pHout) using a micro pH electrode for measurement of pHout.
Similar(58)
The slice culture assemblies were then placed in a microincubator (#PDMI-2; Medical Systems) aerated with 5% CO2-balance air, at 36°C and pH maintained at 7.3, verified using a micro-combination pH electrode (#M-413; Microelectrodes).
After buffer exchange with 50 mM sodium acetate buffer, pH 7.4, using a Micro Bio-Spin chromatography column (Bio-Rad) 1.5 μM MPO was treated with 1.5 mM hydrogen peroxide and incubated at room temperature for 2 h.
All patients were selected from Data set 1. The frozen tumour tissue sections were cut into pieces of ∼20 mg, after which they were solubilized in 800 μl SDS sample buffer (containing 62.5 mm Tris-HCl, 2% w/ v SDS, 10% v/ v glycerol, 0.0025% bromphenol blue, 100 mm DTT, pH 6.8) using a micro grinder.
The peptides were then dialysed for 16 h into 50 mM Tris/HCl, pH 7.5, 150 mM NaCl and 2 mM DTT using a Micro DispoDIALYZER with a 100 Da molecular-mass cut-off (Harvard Apparatus).
The annealed primer-template was separated from unincorporated radiolabeled TTP using a Micro Bio-Spin 30 column (Bio-Rad), which was pre-equilibrated with 20 mM Tris, pH 8.0, 100 mM KCl.
Samples were applied using a Micro dispenser (DRUMMOND).
Peptide concentration was determined using a micro bicinchoninic acid assay (Micro BCA Assay; Thermo Scientific).
Oxygen reduction reaction (ORR) on Pt microelectrode was used for developing a micro pH sensor for scanning electrochemical microscopy (SECM) study in this work.
To the peptide solutions, 0.9 molar equivalents 65CuCl2 was added, the pH was measured using a micro-probe (Hanna Instruments, Italy) and adjusted using concentrated NaOH or HCl.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com