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The present study further characterizes SUMO-1 when compared with SUMO-2/3 in human male meiosis by using a meiotic event, namely the formation of the SC, as a developmental guide.
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cDNA of candidate genes was amplified by PCR using a meiotic cDNA library (kindly provided by Hiroshi Nojima, Osaka University) as template.
A meiotic event should have generated a c.844_845ins68 chromosome lacking the c.833C mutation.
In a novel environment, a meiotic event between two such strains would disperse multiple adaptive alleles (Charlesworth 2012).
Those observations directly link the testis-biased X chromosome deficiency to a meiotic event as expected by MSCI in males.
This would inactivate all copies of a transposon during a meiotic event that appears to be necessary for survival over the hot summer [ 30].
This is the first attempt, using a simple cytological approach, to study the meiotic events in male M. reevesi.
Recent studies in humans have examined the meiotic spindle using a polarized microscope apparatus, which allows the visualization of the polymerization of the meiotic spindle after vitrification and warming.
Each gamete transmitted to the offspring was simulated as an independent meiotic event.
We retained only one germinated ascospore per ascus, to ensure that each progeny was generated by an independent meiotic event and to prevent the collection of sister ascospores.
We experimentally validated six predicted meiotic genes using a genetically tractable yeast system, all of which exhibited sporulation defects.
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