The image was processed using the LASAF software using a median noise filter with 3 iterations at 3 pixels.
To determine synergy between two drugs, the software uses a median-effect method that determines if the drug combination produces greater effects together than expected from the summation of their individual effects.
Medians were compared, and unpaired Student t tests using a statistical software program (GraphPad Prisma 3.0) were used to analyze the lymphotoxin alpha.
Use a free software web browser.
Use a media converter software.
Use a photo collage software tool.
Classes online use a variety of software.
Microarrays were scanned using a GenePix 4000B scanner and software (Axon GenePix 6.0) and normalized to obtain a 635/532 nm ratio (of medians within arrays) between 0.90 and 1.1.
Median Fluorescent Intensity (MFI) data were analyzed with ExPONENT software using a 5-parameter logistic curve-fitting method for calculating cytokine/chemokines concentrations in samples.
Slides were scanned using a Tecan LS reloaded scanner, and median spot intensity was determined using ImaGene software (BioDiscovery Inc., Hawthorne, CA, USA).
After 30 minutes of incubation and extensive washing, the median fluorescence intensity (MFI) of 1 × 104 cells was recorded for every sample using a FACS Canto II flow cytometer and FACS Diva software (both from BD Biosciences, Vienna, Austria).
