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Lung extracts were prepared using a mechanical tissue grinder with 1ml of PBS per each lung and viral titers were determined using plaque assay in MDCK cells as previously described [25], [26].
Tissue was then homogenised using a mechanical tissue homogenizer at 0°C.
Tissue was ground using a mechanical tissue homogenizer and solubilized in lysis buffer provided in the RNeasy Mini Kit (Qiagen USA).
Aortic tissue was ground using a mechanical tissue homogenizer and lysed in a buffer containing 50 mM Tris-Cl, pH 7.4, 1 mM EDTA, 1% Nonidet P-40, 0.1% SDS, 150 mM NaCl, 0.5% sodium deoxycholate, and Halt protease and phosphatase inhibitor cocktail (Pierce Biotechnology, Rockford, IL).
Frozen liver samples were homogenized in chilled 50 mM Krebs-Ringer phosphate buffer, pH 7.4, containing 0.1% Triton X-100, 2.5 mM mercaptoethanol, 0.1% dextran, 5 mM Na2-EDTA, and 0.5% bovine serum albumin using a mechanical tissue disruptor (IKA, Staufen, Germany).
Similar(55)
The tissues were homogenised on ice using a mechanical homogeniser (100 mg of tissue in 1 ml) in 50 mM potassium phosphate buffer (KH2PO4 with 1 mM EDTA, pH 7.4) for assessment of catalase (CAT) activity and in phosphate buffered saline (PBS, pH 7.2) for other parameters.
In order to scan a larger field on the ear, we used a mechanical translating stage to move the tissue sample to the desired location and after acquisition and processing, the mosaics were stitched together to form a larger image.
Use a mechanical pencil.
Use a mechanical system.
You could use a mechanical pencil.[8].
Hearts were disrupted by mechanical tissue disruption using a tissue grinder (Polytron).
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