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Shoreline depth at each pond was measured in May and June 2009 (to the nearest 0.01 m) at three randomly chosen, approximately equidistant, sites (using a measuring band with a weight attached to assure a straight line).
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The Multi-Gauge V 3.0 image analysis program (Fujifilm, Tokyo, Japan) was used to measure band density.
ImageJ software (NIH, Bethesda, MD) was used to measure band densities of scanned blot images.
Quantification was carried out by measuring band intensity using the ImageQuant software (Molecular Dynamics).
Quantifications were performed by measuring band intensities using Image J analysis software.
RNA levels were determined using ImagQuant software to measure band intensity.
Optical density was measured using a wide band (450-580 nm) filter.
The transmission of white light was measured using a broad band white light source (DT-Mini-2-GS, Micropack, Ocean Optics, the Netherlands) that was fibre coupled to a collimating set of lenses in front of the human lens.
DAPI fluorescence was measured using a 463/39 nm band pass filter, FITC fluorescence was measured using a 530/30 nm band pass filter with a 555-nm dichroic long pass filter, CY3 fluorescence was measured using a 580/30-nm band pass filter with 605-nm dichroic long pass filter and CY5 fluorescence was measured using a 650-nm long pass filter with a full mirror.
Intensity of pHrodo fluorescence was measured with a 585/42 (564–606 nm) band pass emission filter and collagen-coated FluoSpheres and DQ-ovalbumin-BODYPY FL-dye were measured using a 530/30 (515–545 nm) band pass emission filter.
Fluorescence emission levels were measured using a band pass filter at 530/30 nm (FI).
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