Sentence examples for using a mass tolerance from inspiring English sources
MS/MS spectra were searched using the SEQUEST search algorithm (version 27, revision 12) against an Mtb protein database [24] using a mass tolerance of 50 ppm and a static modification of 57.02146 Da (carboxyamidomethylation). The search parameters for post-translational modifications comprised dynamic modifications of 243.085522 Da on Lys (GGE) and 15.99491 Da on methionine (oxidation).
Data generated were screened in databases using a mass tolerance ⩽20 ppm.
Data generated were screened in a protein sequence database (NCBInr, current version) using a mass tolerance ⩽20 p.p.m.
Using a mass tolerance of 3 p.p.m., ∼6% of the remaining features were not matched to any unique MFs in the PubChem reference file (301507 entries).
The search parameters specified N-glycosylated and O-glycosylated proteins, with modifications of oxidized methylation and cysteine-treated iodoacetamide, using a mass tolerance of 0.1 Da.
Component detection was performed using a mass tolerance of 10 part-per-million (ppm) and a retention time window of 2.5 min.
The initial precursor mass tolerance was set to 6 ppm, for fragment ions we used a mass tolerance of 20 ppm.
The human metabolome database (HMDB; www.hmdb.ca), the MassBank high resolution mass spectral database (www.massbank.jp), the NIST/EPA/NIH mass spectral library, and the MetaCyc database (http://metacyc.org) were used with a mass tolerance ranging from 0.1 to 0.01 Da for the metabolite searches and identifications.
A reject mass list of 500 ions was used at a mass tolerance of ±5 ppm.
A reject mass list (500 ions) was used with a mass tolerance of ±5 ppm consisting of protonated 2′-deoxyribonucleosides and protonated 2′-deoxyribonucleoside artifacts as listed in Table 1 and the most intense peaks observed in the full scan (250 600 amu) mass analysis over the total chromatographic time period of a sample preparation blank.
Monoisotopic mass values with an unrestricted protein mass using a peptide mass tolerance of ±100 ppm were used for identification.
