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This paper presents an approach to intensify a reactive distillation (RD) process for ethyl acetate (EtAc) production using a mass separation agent (entrainer), and a new process flowsheet with a sidedraw to the RD column.
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This paper presents a novel approach in reactive distillation (RD) processes, the use of a Mass Separation Agent (entrainer).
To overcome this limitation, we have used a mass spectrometry-based approach centered on prior separation of the mucins from the majority of the other proteins.
Samples were assayed by LC-MS/MS using a 2690 separation module with a Quattro Ultima triple quadrupole mass spectrometric detector (Waters-Micromass).
Mass spectra (TOF-ES) were recorded using a Waters 2795 Separation Module and Micromass LCT platform.
Samples were analyzed by HPLC-MS using a Thermo Finnigan TS Quantum mass spectrometer coupled to a Waters 2694 HPLC separations module, 2996 photodiode array detector, and 600 controller, using a Phenomenex C18 column (10 cm × 4.6 mm, 5 μm).
The tryptic peptide was analyzed on a Q-ToF Ultima Global hybrid tandem mass spectrometer (Waters) with an online CapLC ternary nanoHPLC system using a 2-hr separation program.
Plasma proteins were analyzed by mass spectrometric analysis using a one-dimensional (1-D) separation approach as described below [ 12].
The system studied is the ethanol/water separation using cyclohexane as mass separating agent.
In short, the final separation and detection were performed by LC MS/MS using a Quattro Ultima tandem mass spectrometer coupled to a HPLC 2690 separation module system and integrated autosampler (Micromass, Manchester, UK).
The peptide digests were analysed by LC MS/MS using a nano-scale reverse-phase separation column (75 μmm100 mm; Nano-Separations) and an LTQ OrbiTrap XL mass spectrometer (Thermo Fisher).
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