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The secondary antibody was goat anti-mouse (FITC; Santa Cruz, CA, USA); neural differentiation was evaluated using a marker of neuronal class III β tubulin, Tuj-1, a mouse anti-human antibody (Covance, Berkeley, CA, USA) and anti-Brn 3a (a transcription factor), that is a mouse anti-human antibody (Santa Cruz, CA, USA).
Using a marker of endocytosis, the styryl dye FM1-43, this report shows that rapid apical endocytosis in zebrafish lateral line sensory hair cells is calcium and calmodulin dependent and is partially blocked by the presence of amiloride and dihydrostreptomycin, known inhibitors of mechanotransduction channels.
These studies reflect the range of resolutions at which information can be acquired using a marker of this type.
Immunohistochemical analysis using a marker of hypoxia revealed that the radiation-induced reoxygenation continued until at least 24 h postirradiation.
To examine this possibility, we performed an immunohistochemical analysis using a marker of hypoxia, pimonidazole (Kennedy et al, 1997; Figure 1C and D).
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What to do: Use a marker to line the rim of the top of the pumpkin.
Since vimentin is used as a marker of mesenchymal cells, we next used a mesenchymal stem cell marker STRO-1.
The use of anti-TNF α therapy was used as a marker of disease severity.
The expression of K19 was used as a marker of intermediate differentiated cells, and the expression of K8 was used as a marker of luminal epithelia (21, 22).
Breast density, which is increasingly used as a marker of breast cancer risk, is associated with the use of multivitamins, a new study shows.
Synaptophysin was used as a marker of synapse to detect synaptic connections between newly formed neurons.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com