Sentence examples for using a manual micropipette from inspiring English sources
Baseline fluorescence was taken every 15 seconds for 2 minutes prior to addition of the NMDAR agonist using a manual micropipette.
I used a manual typewriter.
For instance, Sinigalliano et al. [ 21] compared the isolation efficiency of dinoflagellates between FACS and manual picking using a micropipette and the former approach appeared to be less efficient than the latter (0.5% vs 2%, respectively).
Indeed, because we have found that the magnetic "pullout" protocol is not 100% efficient (Mendes et al. in preparation), a third incubation with control serum was conducted, but in this case, IgG-binding beads were visualized by incubating the beads with red quantum dots (QDot655), permitting visual inspection of the hit beads followed by manual removal using a micropipette.
Patch pipettes were prepared from borosilicate glass capillaries (G150F-3; Warner Instruments, Hamden, CT) using a P-1000 micropullere puller (Sutter Instruments, Novato, CA) and were subsequently fire polished.
Thromboplastin (25 µl) was added to all wells simultaneously using a multichannel micropipette.
The cells were mechanically dissociated using a Gilson micropipette, filtered through 48 μm diameter pores and centrifuged again.
Peripheral blood was collected from the mouse tail using a heparinised micropipette tip.
The dissociation of cells was completed mechanically using a glass micropipette by pipetting up and down.
