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Core biopsies (1 mm) of paraffin embedded tumor areas were incorporated into a tissue microarray with 1.5 mm between core centres using a manual arrayer (MTA-II, Beecher Instruments, WI, USA).
The TMAs were constructed using a manual arrayer (Beecher Instruments) with tissue core diameters of 0.6 mm.
TMAs were made in triplicate using a manual arrayer (Beecher Instruments, Sun Prairie, WI, USA) with 0.6 mm stylet.
Five paraffin-embedded tissue microarray blocks of normal and tumor tissue specimens obtained from these patients were created using a manual arrayer (Beecher Instruments, Sun Prairie, WI, USA).
Three cores per patient with a diameter of 0.8 mm were chosen and transferred to a paraffin block using a manual arrayer (Beecher Instruments, Sun Prairie, WI).
Tissue micro arrays (TMAs) containing two 1-mm-diameter cores from each tumour were constructed using a manual arrayer (Beecher Instruments, Silver Spring, MD, USA).
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TMAs were constructed using cores selected from FFPE blocks of primary OSCC tissue, using a manual tissue arrayer (MTA-1, Beecher Instruments, Sun Prairie, WI, USA) as previously described (Parsons and Grabsch, 2009).
Briefly, two 0.6 mm diameter tissue cores were collected from each tumour block and arranged in a recipient block using a manual tissue arrayer (Beecher Inc., Sun Prairie, WI).
For each tumor, three core biopsies 1 mm in diameter were then taken from selected areas of donor blocks and arrayed into a recipient paraffin block using a manual tissue arrayer (Beecher instruments, Sun Prairie, USA).
Benign and malignant tissue specimens from a consecutive series of 448 patients undergoing radical prostatectomy for localized prostate cancer at Malmö University Hospital, Sweden, between 1988 and 2003 were mounted in 17 tissue microarray blocks using a manual tissue arrayer (Beecher Inc ,Wisconsin WI) as previously described[39].
TMAs were then constructed using a manual tissue arrayer (Beecher Instruments Inc., Sun Prairie, WI, USA).
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