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Prior to the adhesion measurements, the sample was aligned using a manual alignment stage and applying the alignment process published by Kroner et al. Force and displacement were recorded during all measurements.
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Kinannote uses an HMM derived from a manual alignment of the Dictyostelium kinome (Goldberg et al. 2006).
The CR data was initially aligned using CLUSTAL X [52] and subsequently both datasets were optimised by visual inspection in a manual alignment editor [53].
Because Vinick uses a manual typewriter".
I used a manual typewriter.
Use a manual camera.
Use a manual juicer.
An amino acid residue consensus was also determined for each SMR subclass from our alignment and the results are presented as percent occurrence in Figure 5. SMR proteins alignments to BAT, DMT and MFS transporters were performed using ClustalW and manual alignment using the editing program GeneDoc (v 2.5.010 [ 64]).
Using GORAP and manual alignment correction one PyrR-binding site was identified in each isolate, which is located upstream of a variety of genes involved in pyrimidine biosynthesis.
For this approach, a user will provide an IFC file, outputs from one of the the three 3D reconstruction tools, and values of Origin, Rotation, and Scale (used during manual alignment and as shown in Fig. 5).
BioEdit (Hall 1999) was used for manual alignment and confirmation of annotations.
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