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CD34+ HSPC were purified using a magnetic bead isolation step from patients undergoing autologous stem cell transplantation.
Samples were washed and CD4+CD25+FOXP3+ Tregs were isolated based on their surface expression of CD25 using a magnetic bead based isolation kit (Miltenyi Biotec, Inc).
The population of Treg was depleted using two different methods: in three subjects, we removed the CD4+CD25+ Treg using a magnetic bead separation system (AutoMACS cell separation, Miltenyi Biotec), as per manufacturer's instructions, while in the other 4 subjects, we used a flow-based cell sorter (Beckman Coulter MoFlo) to separate the CD4+CD25hi T cells.
Viral nucleic acid was extracted using a magnetic bead separation method and subsequently amplified with the consensus primer PCR.
Immunoprecipitation (IP) of cystatin C from CSF was performed using a magnetic bead system (MACS Molecular, Auburn, CA).
DNA from the parents was isolated from peripheral blood leucocytes using a magnetic bead method and the QIAsymphony automated extraction system (Qiagen).
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This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157 H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40 60 min.
pMACS Kk.II-transfected cells were enriched using a magnetic bead-tagged antibody to human H2Kk and a MACS apparatus (Miltenyi Biotec Inc).
MALDI-TOF MS samples were processed using a magnetic bead-based weak cation exchanger (WCX, Bruker Daltonics, Germany) according to the manufacturer's protocols.
Insulin was analyzed using a magnetic bead-based metabolic panel kit from Millipore, Billerica, MA, USA (Cat# HMHMAG-34 K-06).
In the present study, we investigated differences in the salivary peptide (1 10 kDa) profiles of T1 stage OSCC patients and healthy subjects by MALDI-TOF MS using a magnetic bead-based peptidome analysis of saliva samples.
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