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Thus while a small experiment using a low starting quantity of RNA may fail to identify many subtle changes, one can have confidence in any changes that are reported.
In a phase I study using a low starting dose of 2.5 mg, and escalating 5 mg each 28 days to 10, 15, and 20 mg, one-third of patients could not escalate beyond 2.5 mg, although those who reached 20 mg did not suffer any dose-limiting toxicities [ 22].
Plasma DNA also shows a higher number of PCR duplicates compared to cellular (3 % vs. 0.5 %), which is expected, given the extra PCR cycles in the library protocol when using a low starting volume of DNA.
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The overarching principal, as in other areas of geriatric medicine, is to use a low starting dose and to slowly increase the dose as necessary.
Our previous experience prescribing topiramate led us to use a low starting dosage and a slow dosage-escalation protocol.
Using a low-resolution filtered starting model, we then carried out projection matching and angular reconstitution cycles (see 'Materials and methods'; Figure 5 figure supplement 1C, Table 4) and then refined a 24 Å TBC-DEG:αβ-tubulin map.
Try using a low chair.
Using a low grade detergent.
Beat slowly or using a low speed.
We compared the same RNA sample at different dilutions, using the "low starting material" amplification procedures to obtain labelled cRNA for the low concentrations (see Material and methods).
Also, Wendy's animal welfare council said that one of its chicken suppliers has started using a low-atmospheric pressure system that renders chickens unconscious before the birds are handled by plant workers, replacing the industry standard practice of stunning chickens with electricity.
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