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A second method, using a low pH LC separation, was designed to provide higher chromatographic resolution, facilitating identification of metabolites.
Mammary gland sections were rehydrated and subjected to antigen retrieval using a low pH citrate buffer at 37°C, quenched for endogenous peroxidase activity with 3% H2O2 in methanol for 10 min at RT, and incubated at 4°C overnight with a primary antibody against TrkB at 1∶400 (Santa Cruz Biothechnology, Santa Cruz, CA).
For example demonstration for the importance of ion homeostasis and intracellular pH maintenance raises the possibility that Directed Evolution could be used to generate new strains using a low pH environment as the selective pressure.
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All solutions were filtered through 0.10 μm pore-size filters using a low vacuum system before initial pH measurement and refrigeration.
Also, the use of a low pH for efficient ESI-MS in positive ionization mode is not an option when studying biological noncovalent complexes, nor is the commonly used high percentage of organic modifier.
Perchloric acid was used to obtain a low PH to promote ionization of metallic atoms into cations instead of oxides.
DNA was eluted from the beads using a low-salt (10 mM Tris-HCl; pH 8.5) buffer.
Simultaneous extraction of HM's and recovery of P from wastewater or raw sludge using 3C ED was be most effective at a low pH using anaerobically digested sludge.
Use a low nitrogen, neutral pH fertilizer and apply as instructed on the packaging.
The robustness of a generic method for chiral separation in capillary electrophoresis using highly-sulfated cyclodextrins in a low pH phosphate buffer and the "short-end injection technique" was studied.
As clathrin-coated or other vesicles used for endocytosis are characterized by a low pH, this provided us with a tool to visualize internalization of the conjugated anti-HER2 antibody.
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