Attempts to amplify the genome in several 5−6 kb pieces using a long- polymerase chain reaction (PCR) protocol (Burger et al. 2007) were unsuccessful due to the relatively poor quality of the DNA template.
The PCRs were performed using a long-range polymerase (LA Taq™, TaKaRa Bio Inc, Shiga, Japan).
We identified novel structural variations (SVs) between L er-0 and the reference accession Col-0 using a long-range polymerase chain reaction approach to generate an Illumina data set that has positional information, that is, a data set with reads that map to a known location.
Statistical comparisons were made with ANOVA tests, using Statistica software (StatSoft, Inc .. To generate the p[hmgcr-GAL4] lines, we amplified by PCR, two DNA fragments from wild-type CS, using a long range Taq DNA polymerase (Invitrogen Long Range) according with the fabricant protocol.
PCR analyses that bridged the genomic breakpoints were performed by using a long-range PCR polymerase mix (Fermentas, St Leon-Rot, Leon-Rot, Germanyg to the maccordinger's descriptoon.
Partial sequences of the mitochondrial genomes were determined for two calanoid copepods, Eucalanus bungii [9530 bp (base pairs)] and Neocalanus cristatus (7965 bp), using an approach that employs a long polymerase chain reaction (PCR) technique and primer walking.
Use a long handled spoon.
Using a novel long quantitative polymerase chain reaction (QPCR technique), our laboratory demonstrated that mtDNA is damaged early in EAU (day 4 postimmunization).
