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Using a long pipette, the technician distributed the slurry among five sterilized plastic bottles.
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After the two phases became clear and transparent and the interface was well defined the bottom phase was carefully removed using a long needle syringe and a pipette was used to remove the top phase.
Use a long ladder.
Use a long putty knife.
Use a long handled spoon.
Never use a long eraser.
Excess liquid was removed using a transfer pipette fitted with a 200-μL pipette tip.
It is immobilized using a holding pipette.
Cells were incubated until 85% confluency and then wounded using a yellow pipette chip.
A scratch was made in the monolayer of transfected keratinocytes using a P10 pipette tip.
After cells reached 90% confluence, cross lines were generated using a sterile pipette tip.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com