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The evolutionary age of each subfamily was determined using a local nucleotide substitution rate derived from WSINE1 (Additional file 1: Table S4).
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The haplotype associated mapping (HAM) algorithm uses ANOVA to calculate the strength of genetic associations between an input phenotype and the ancestral haplotype structure (as inferred using a local window of three adjacent single nucleotide polymorphisms (SNPs) across the genome).
Nucleotide alignments were generated using a local BLAST v 2.2.22 installation which were then visualized and analyzed for gene conservation and sequence synteny using the Artemis Comparison Tool (ACT) [ 76].
Sequences were compared for identification to their closest matches in GenBank using the NCBI nucleotide Blast search, and to unpublished sequences using a local blast search in the laboratory of R. E. Ricklefs.
Using a local installation of the BLAST application (version 2.2.14), a homology search of the reference ABC transporter nucleotide sequences was performed against the human genome masked for ABC transporter exons.
Nucleotide sequences of the contigs were blasted against the Solanaceae Genomics Network (SGN) tomato database for annotation, using a local E-Blast tool; 3,295 contigs were not annotated.
Nucleotide sequences of the contigs were then blasted against the SGN unigenes v2 tomato database (ftp.solgenomics.net/unigene_builds/combined_species_assemblies/tomato_species) for annotation, using a local Eblast tool (E value 1e 9).
Single nucleotide exchanges and indels based on dbSNP version 129 [ 41] were assigned to the coding exons of all RefSeq genes using a local installation of the University of California Genome Browser and the associated tool kit [ 35] and analyzed with custom Perl scripts.
We used a local BLASTx query [ 61] on a database of HIV-1 subtype B and C reference samples to translate the nucleotide fragments into their corresponding protein sequences (see Additional file 1).
Use a local neighborhood watch program.
Comparison of blastn (search a nucleotide database using a nucleotide query) and tblastx searches (search translated nucleotide database using a translated nucleotide query).
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