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Samples were collected from the receiver chamber at regular intervals up to 4 h during the experiment (with or without US), and tracer molecule concentration was determined using a liquid scintillation counter (Packard, Meriden, Connecticut, USA).
The experiment was performed using a liquid scintillation detector.
Bound radioactivity was quantified using a liquid scintillation counter.
The radioactivity was quantified using a liquid scintillation counter.
Bound radioactivity was measured by scintillation counting using a liquid scintillation cocktail.
Membranes were washed and filtered, and radioactivity was quantified using a liquid scintillation counter.
The radioactivity of the beads was measured using a liquid scintillation counter.
Samples were left in the dark at room temperature for 4 h and then counted using a liquid scintillation counter.
The amount of radioactivity incorporated was determined by using a liquid scintillation beta counter (Beckman Instruments, Inc.Madrid, Spain).
The ionizing radiation of each probe was measured using a liquid scintillation counter (L2 6500, Beckman Coulter, Fullerton, CA).
Mitochondria were lysed with 50 µl of 1% SDS, and [3H] ADP uptake was measured using a liquid scintillation counter [17].
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