Using a linear 15 95% acetonitrile gradient, we were able to separate all five matching pairs of PCB sulfates from their OH-PCB precursors [see Supplemental Material, Figure S3 (http://dx.doi.org/10.1289/ehp.1206198)].
Fractions were eluted using a linear gradient (0 100%, v/v) of acetonitrile (solvent B) at a constant flow rate of 1.0 mL/min over 40 min.
Radiotherapy was delivered using a linear accelerator (CLINAC C2100, Varian) with a 6 15 MV photon beam up to a median dose of 59.4 Gy and 1.8 Gy as daily fractionation.
The enzyme solution was applied to a hydroxyapatite column equilibrated with 1 mM phosphate buffer (pH 7.0) and PGA was eluted using a linear gradient 0-100% of 200 mM phosphate buffer.
Peptides were eluted using a linear gradient of 10 35% buffer B over 60 minutes, followed by isocratic elution at 80% buffer B for 5 minutes with a flow rate of 0.25 µl/min across the column.
The column was eluted using a linear NaCl gradient (0 200 mM), pink fractions were collected and concentrated using a centricon (5 kDa cutoff).
The elution was carried out using a linear gradient of 0 100% (99% acetonitrile plus 0.1% TFA) at a flow rate of 0.75 mL/min.
The elution was carried out using a linear gradient of 30-70% acetonitrile at a flow rate of 0.8 ml/min in the presence of 0.1% trifluoroacetic acid (TFA).
The elution was made at room temperature using a linear gradient from 10 60% of acetonitrile in trifluoroacetic acid (0.05% v/v) at a flow rate of 1.0 mL/min in 30 minutes.
Peptides were autosampled onto the packed column at a flow rate of 500 nl/min and were separated over 20 min using a linear gradient of 13 34% (v/v) acetonitrile/0.5% (v/v) acetic acid.
The denatured domains were further purified by RP-HPLC (reverse-phase HPLC) on a Discovery wide-pore C18 column (21.2 mm×150 mm), using a linear gradient of 10 60% acetonitrile in 0.1% TFA (trifluoroacetic acid) over 60 min at 4 ml/min.
