Sentence examples for using a linear salt from inspiring English sources

Classical ion-exchange chromatography using a linear salt gradient to elute the adsorbed protein at fixed pH is the most common method to separate product-related impurities during downstream processing of biopharmaceuticals.

The protein was eluted using a linear salt gradient of buffer B supplemented with 2 M KCl.

Bound protein was eluted from the column using a linear salt gradient of 0 - 1 M NaCl.

Protein was eluted using a linear salt gradient in buffer A from 100 mM to 500 mM NaCl over 20 mL with 0.5 mL fractions.

The peptides were eluted using a linear salt gradient (0 to 35%) of solvent B (10 mM potassium phosphate buffer containing 30% ACN, 350 mM KCl, pH 2.7) at a flow rate of 200 μl/min.

A second purification was performed under conditions previously described [ 52] using pH 8.0 and a linear salt gradient (20 mM – 1 M KCl) at 4 °C by non-denaturing FPLC cation exchange chromatography.

Separation was carried out using Äkta FPLC (GE Healthcare) with a linear salt gradient (0-1 M NaCl) in the loading buffer.

S15 was soluble and was purified at 4 °C using non-denaturing FPLC cation exchange chromatography, pH 5.5, with a linear salt gradient (100 mM-1 M NaCl)[ 51].

Further protein purification was achieved using a HiTrap S-HP column (5 mL) (G.E. Healthcare, QC, Canada) and a linear salt gradient.

Proteins were eluted in 10 column volumes in a linear salt gradient to 0.25 M NaCl.

Proteins were eluted in a linear salt gradient from 0 to 70% in 7 column volumes.