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Inter- and intraparticle mass transfer was represented by a lumped overall mass-transfer coefficient using a linear concentration driving force.
Adsorbed proteins were eluted using a linear concentration gradient of NaCl (0 0.5 M) at a flow rate of 0.5 ml/min.
His6-tagged TssK was eluted using a linear concentration gradient of 0 500 mM imidazole in IMAC elution buffer B (50 mM Tris/HCl, pH 7.5, 250 mM NaCl and 500 mM imidazole) over 12 column volumes.
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The cleavage mixture of ZIKV helicase180 617 was loaded onto a HiTrap S 5 mL column (GE) pre-equilibrated with buffer C (50 mmol/L HEPES, pH 7.0, 50 mmol/L NaCl and 5% glycerol) and also eluted using a linear NaCl concentration gradient.
The supernatant was inserted into a Protein Pack SP 5PW column (0.78 x 7.0 cm) and the elution performed using a linear gradient of concentration between 0.05 and 1.0 M ammonium bicarbonate with 750 μL of 0.1% (v/v) trifluoroacetic acid (Buffer B).
Taste sensitivity (intensity scaling with suprathreshold concentrations) was estimated with different linoleic acid concentrations using a linear scale of 150 mm labeled at the ends.
Protein was eluted using a linear gradient of increasing imidazole concentration.
To quantify this effect, each object was reconstructed and the resulting intensity was converted to concentration using a linear fit to the dilution series data.
There is fear about the risk of lung cancer due to inhaled radon gas because of the widespread publicity given to the studies that link lung cancer incidence to radon concentration using a linear no-threshold (LNT) model.
By calculation of the natural logarithms of metal ion concentrations, they were transformed to normal distributions and metal ion concentrations were therefore subsequently analyzed using a linear model with cobalt or chromium concentrations after 6 years as the dependent variable, the type of bearing as the fixed factor, and preoperative cobalt or chromium concentrations as a covariate.
The plasma input function was modeled using a linear interpolation of the [11C]befloxatone concentrations before the peak and a tri-exponential fit of concentrations after the peak.
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