Sentence examples for using a linear ampLIFication from inspiring English sources
Biotin-labelled cRNA was produced using a linear amplification kit (Ambion, Austin, TX, US) with 400 ng of quality-checked total RNA as input.
Biotin-labelled cRNA was produced using a linear ampLIFication kit (Ambion, Austin, TX, United States).
Biotin-labeled cRNA was produced using a linear amplification kit (Ambion; Austin, TX, USA).
The ds cDNA is then amplified using a linear amplification procedure.
Total RNA was extracted from human xenograft tissue and amplified using a linear amplification process.
We developed a method to prepare and label miRNA targets using a linear amplification procedure in which an antisense-labelled target is produced.
We used 60°C extension temperature, and Bartfai et al. [ 19] used a linear amplification method that avoided biased sequencing of the AT-rich Plasmodium genome; these two approaches therefore produced similar numbers of junction calls.
Total RNA was prepared using Qiagen (Chatsworth, CA) RNeasy kits and amplified using a linear T7 promoter-based mRNA amplification method incorporating amino -allyl dUTP followed by random primer labeling with Cy™3 or Cy™ 5 (Amplification and labeling kits are available from Ambion, Inc., Austin, TX).
We found a good correlation between AVs30s and amplification values using a linear regression.
Bacterial-RNA samples were amplified using a RNA linear amplification kit SenseAMP (Genisphere), as described previously [75], [77].
In brief, 0.5 μg of total RNA was amplified using a Fluorescent Linear Amplification Kit (Agilent Technologies) and labeled with Cy3-CTP or Cy5-CTP (CyDye, PerkinElmer, CA, USA) during the in vitro transcription process.
