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Herein, we present such de-cross-linking features using a light sensitive cross-linked polymeric nanoparticle system.
Blots were viewed with a chemiluminescent substrate (SuperSignal West Pico Chemiluminescent Kit, Pierce) using a light sensitive film (Amersham Hyperfilm ECL, GE Healthcare) or FluorChem Imager (Alpha Innotech Corp) and FluorChem IS-8900 software.
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While the company worked hard to make it as easy-to-use as possible the system still uses a light sensitive resin that must be cured in isopropyl alcohol, a process that is at once messy and potentially hazardous.
Roska's group targeted the ON bipolar cells, using a light-sensitive algal protein called channelrhodopsin-2 (ChR2) to confer light sensitivity where there previously was none.
The researchers could induce the opposite effect using a light-sensitive protein that silences the cells instead of activating them.
One example exists in which the spectral properties of the gel can be altered using a light-sensitive gelator, with a gel being maintained throughout22.
Once the implant is in place, visual information is captured using a light-sensitive chip, much like that found in a digital camera.
Instead, this mouse's ON bipolar cells another retinal cell type, shown in green have been engineered to detect and respond to light using a light-sensitive channel protein normally found in algae.
The acute light response in activation of PRR9, LHY/CCA1, GI, transcription (eqs. 5, 1, 27) was modelled using a light-sensitive activator – protein P (c P ), which is accumulated in darkness and was degraded in light similarly to the P2011 model (eq. 4).
The charge-coupled device (CCD) uses a light-sensitive material on a silicon chip to electronically detect photons in a way similar to the photomultiplier tube.
For his demo, Riedel-Kruse uses a light-sensitive organism call Euglena and surrounds the slide with four LEDs that can be controlled with a joystick (after kids do a bit of work assembling a circuit to connect the joystick to the LEDs).
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