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Cultures were incubated at 37°C, 5% CO2, and quantified based on morphological recognition using a light microscopy as described by Stem Cell Technologies.
Two independent observers evaluated the sections using a light microscopy Leica DM LS (Leica Microsystems; Wetzlar, Germany).
The slides were assessed using a light microscopy coupled to the photomicrographic camera, both adapted to a microcomputer with software Honestech for image capture.
Samples were transferred into chloral hydrate solution (2.5 g mL-1) and boiled about 20 min. After several exchanges of hydrate chloral solution, samples were equilibrated in 50% (w/v) glycerol and observed using a light microscopy.
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Second, an RNAi screen was performed to investigate the role of these proteins in the organisation of the secretory pathway using a light microscopy-based assay (Figure 1 and 8).
Costs were collected for testing conducted using Xpert, MTBDR plus, Ziehl-Neelsen smear microscopy using a light microscope, and florescence smear microscopy using auramine-O staining and a light emitting diode microscope [ 9].
Slides were evaluated by two independent investigators (K. S. and H. T). in a blinded fashion using a light microscope, a Zeiss Axioscope (Carl Zeiss microscopy GmbH, Jena, Germany).
Sputum-smear microscopy using a light emitting diode (LED) fluorescence microscopy was done at Gondar University Hospital Laboratory on the day of collection following the manufacturer's procedures (PARTEC GmbH).
Sections were scanned using a.slide light microscopy slide scanner at 100 × magnification (Olympus, Hamburg, Germany) and analysed entirely using Leica QWin Pro version3.5.1 software (Leica Microsystems, Cambridge, United Kingdom) by a blinded observer.
The CISH signals were seen as dark brown dots and counted in at last 100 nuclei with a light microscopy using a × 40 objective.
HFF-MBPsi-4 diaplayed increased in size as compared to HFF-control cells (Fig. 2, panel A) using light microscopy under same magnification.
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