Sentence examples for using a light Cycler from inspiring English sources

The PCR products were amplified from the single-stranded cDNA as the template and quantified using a Light Cycler 480 (Roche Diagnostics K.K., Tokyo, Japan) according to the following program: 10 s at 95 °C, followed by 50 cycles of 95 °C for 5 s and 60 °C for 34 s.

Quantitative RT PCR was performed using a Light Cycler (Roche) and the Invitrogen kit (SYBR®).

q RT PCR was performed using a Light Cycler (Roche) and the Invitrogen kit (SYBR®).

HIF-1α, VEGF and actin gene expression was quantified using a Light Cycler 480 System (Roche Diagnostics, Mannheim, Germany).

Quantitative PCR analysis was performed using a Light Cycler (Roche) according to the manufacturer's instructions using a SYBR Green I kit (Roche).

PCR products were quantified using a Light Cycler 2.0 (Roche Diagnostics GmbH, Mannheim, Germany) according to the following protocol: after heating for 10 min at 95°C, amplification was performed by 40 cycles of 3 s at 94°C, 5 s at 56°C and 20 s at 72°C, followed by a melting curve analysis.

Each sample was analyzed in triplicate using a Light-Cycler 480 System.

Quantitative PCR is realized using a light-cycler (Roche Diagnostics, Germany).

The RT products were used as template for quantitative real-time PCR using a Light-Cycler instrument (Roche).

Quantitative PCR was performed using a Light-Cycler® 480 Real-Time PCR System (Roche, Basel, Switzerland) and the SYBR Green qPCR Master Mix (2X) (Fermentas, USA).

The quantitative PCR analysis was performed using a Light-Cycler 480 Real-Time PCR instrument (Roche Diagnostics, Basel) by the 5′ nuclease assay (Taqman assay).