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Yonezawa et al. [8] described the development of a system consisting of multiple specialized robots that lift automobiles using a lifter.
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Using a jar lifter or tongs, lower sealed jars into water bath.
A portion of the cells on each plate were scraped up using a cell lifter and transferred in PBS to a sterile microfuge tube.
After removing the membrane bound 125I-Ang(1 12), the myocytes were scraped using a cell lifter in 1 mL of acid-ethanol (0.1N HCl + 80% ethanol).
After discarding non-adherent cells and extensive washings, the monocytes were recovered using a cell lifter.
Following 1× PBS wash, cells were manually harvested using a cell lifter.
Cells were collected using a cell lifter (Thermo Fisher, Waltham, MA, USA), transferred to an Eppendorf tube, passed through a pipet tip five times and incubated on ice for 10 min.
Cultures and lrECM were then separated from the dish using a cell lifter, and contents were transferred to a conical tube for an additional 30 minutes on ice to allow full dissolution of the lrECM.
Cells were detached from the culture plate using a cell lifter in the presence of the extraction solvent, and the cell mixture was transferred into a 1.5-mL microtube (Diamed, Mississauga, ON, Canada).
If you're using a root lifter, make sure you only use it on your roots.
Lift them out of the canner using a jar lifter and let them rest on the counter until they come to room temperature.
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