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Efficient cleavage sites in HnudC mRNA were identified using a library selection technique and HnudC-targeted internal ribozymes were cloned into a triple ribozyme cassette.
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An elegant selection screening system using a library of riboyzmes was also able to identify a potential regulator of BRCA1 expression [ 52].
Such a library selection will decrease the practical frequency ratio.
Murphy (2003) used a library function to bring a variation in fitness scaling, selection, and other genetic operators such as crossover and mutation to solve the particular problem.
Anyone can use a library.
Use a library.
We performed growth selections upon an E. coli genome library developed using a moderate selection pressure to identify genomic regions implicated in acetate toxicity and tolerance.
An in vivo selection process using a miRNA library revealed six miRNAs that were shown to confer estrogen-independent growth and increased phosphorylated-Akt in MCF-7 breast cancer cells [ 102].
This information was used as another library selection criterion.
When in-house compound collections are available, they could also be used to bias library selection via two opposite strategies.
In all cases, the multiple small molecules predicted to bind the predicted essential C. difficile proteins could be used to bias library selection for the rational development of new inhibitors.
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