Sentence examples for using a lentiviral system from inspiring English sources

We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold.

To elucidate whether MeCp2 plays a role in hypermethylation at CpG and non-CpG sites in the first exon of the HIF-1α gene, an MDA-MB-231 stable cell strain overexpressing MeCp2 was established using a lentiviral system (Fig. 6c); overexpression efficiency was confirmed by RT-PCR and western blotting.

In addition, AMSBIO scientists can quickly establish an optimized cell line, using a lentiviral system, according to scientists' specification.

BAMBI protein turnover was studied in HUVECs with BAMBI overexpression using a lentiviral system.

We generated BAMBI overexpressing HUVECs using a lentiviral system allowing for a more reliable analysis of BAMBI protein metabolism.

Stable cell lines overexpressing miR-30a and -191 were generated using a lentiviral system as described above.

As the specific band for BAMBI protein was rather faint in the Western blots from mGEC and HUVEC, we used a lentiviral system to overexpress BAMBI in HUVECs.

We next used a lentiviral system of doxycycline-triggered Dll1/DLL1 overexpression to evaluate the effects of gain-of-function in the early differentiation step.

We used a lentiviral system that integrates NF-κB-driven promoters with destabilized green fluorescent protein (dGFP) expression as a reporter into the genome of monocyte THP-1 cells to further validate the transfection-based luciferase activity.

To further exclude effects of DNMT1 or DNMT3b on methylation at CpG and non-CpG sites within HIF-1α gene promoter across TSS, MCF-7 cell strains with stable knockdown of DNMT1 or DNMT3b were established using a lentiviral vector system with GFP (Fig. S3A&B).

Gain- and loss-of-function experiments using a lentiviral expression system showed that Homeobox A2 (Hoxa2) was negatively regulated by miR-135, and luciferase reporter assay further indicated that miR-135 repressed Hoxa2 expression through binding to the 3′-untranslated 3′-untranslated of the Hoxa2 mregion