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In addition, cell diameter was measured using a Leica Light Microscope M125 (Leica Microsystems).
After 18 20h, photos were taken using a Leica light microscope.
RNAi phenotypes were analysed after 48 72 hours at 21°C using a dissecting microscope and by mounting worms on 2% agarose pads which were viewed using a Leica light microscope fitted with Nomarski optics.
To mount adult wings, flies were pre-incubated in 80% ethanol and 20% glycerol solution, then dissected and mounted in 70% glycerol before analysis using a Leica light microscope.
The sections were analyzed using a Leica light microscope.
The CC was traced at × 10 magnification using a Leica light microscope.
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Stained slides were observed under polarized light using a Leica DMBL light microscope microscope equipped with a polarizer/analyzer set.
Histological examinations of tissue sections were performed after hematoxylin-eosin-saffron (HES) staining, using a Leica DMR light microscope (Leica Microsystems, Wetzlar, Germany).
The immunolabeled sections were photographed using a Leica Q550IW light microscope (Leica Microsystems, Heidelberg, Germany).
Section images were captured using a Leica DMRB light microscope equipped with a CoolSNAP digital camera and IPLab software.
Cell counting was carried out in 8 12 randomly chosen fields using a Leica Wetzlar light microscope (Germany) at 400× magnification, 0.180 mm/field.
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