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CO was measured by transthoracic impedance (Bomed NCCOM 3; Bomed, Irvine, CA, USA) using a lateral spot electrode configuration and incorporating the Sramek-Bernstein equation [ 13].
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Sinuses were grafted using a lateral approach.
Using a lateral approach, the right proximal femur was exposed.
By limiting its aperture, we could demonstrate an effective excitation PSF having a lateral spot size of 209±20 nm in vacuum.
Even in the optimal case of diffraction limited imaging and full pupil size of 7 mm one would be limited to a lateral spot size of 3.6 µm.
Standing left to right lateral projections of the abdomen were taken using a focal spot film distance of 200 cm.
Radiographs at the Regional Animal Hospital of Helsingborg were taken with an x-ray tube mounted on an overhead gantry using Kodak digital image plates (Carestream Health, Inc. Rochester, N.Y .. Standing left to right lateral projections of the abdomen were taken using a focal spot film distance of 180 cm.
Pictures were taken using a RTcolor Spot camera (Diagnostic Instrument Inc) with Spot Advance v3.0 software.
Briefly, each protein spot on SYPRO Ruby stained gels was picked using a spot picker (Amersham Biosciences).
Protein spots representing greater than twofold alteration in expression were picked using an Ettan Spot Picker (GE Healthcare Biosciences).
Spots were excised using an Ettan™ Spot Picker robot (GE Healthcare).
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