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MMP activity was quantified using a laser densitometer and expressed as arbitrary units (au).
The autoradiograms were scanned using a laser densitometer (Scion Corporation, Frederick, MD, USA) and the absorbance values of the distinct ADAMTS PCR products normalized relative to the corresponding GAPDH value.
Immunoblot densities were quantified using a laser densitometer.
The immunoblot densities at 130 KD were quantified using a laser densitometer.
The immunoblots of PPAR δ (50 kDa), cardiac troponin I (28 kDa), and phospho-troponin I (28 kDa) were then quantified using a laser densitometer.
The protein pattern was divided into six fractions, albumin and α1-, α2-, β1-, β2- and γ-globulins, using a laser densitometer (Sebia Hyrys2, Sebia, Issy-les-Molineaux, France).
Similar(53)
Using a laser would be easier.
After rinsing twice with TBST and once with TBS (TBST without Tween-20), antibodies were detected with enhanced chemiluminescence was scanned using a Molecular Dynamics laser densitometer and analyzed by NIH Image software.
The dried gel was exposed for autoradiography overnight and the intensities of the S100A4 bands were subsequently analysed using a Personal Laser Densitometer SI (Amersham Biosciences, Sunnyvale, CA, USA).
The relative amount of PEPC protein in clarified extracts from +Pi compared with −Pi cells and seedlings was estimated by quantification of the immunoreactive 107-kDa PEPC polypeptides (p107) on immunoblots by measuring A633 using an LKB Ultroscan XL laser densitometer and Gel Scan software (version 2.1) (Pharmacia LKB Biotech).
The hybridization signals were quantitated by scanning the autoradiograms with a laser densitometer and using the ImageQuant software.
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