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1, 3-Bis-trimethylsilyloxy-2-methylisoindole is formed instead using a large excess of silylating agent.
Due to the symmetry of cyclic DTPA anhydride 1, the di-addition product 3 may form, which is minimised by using a large excess of cyclic DTPA anhydride 1 compared to amine 2. The reaction conditions were optimised for maximising the yield of target linker 4 by modifying a variety of parameters such as concentration and amounts of reactants.
To achieve this second element of specificity, we selected for aptamers with slow dissociation rates (t1/2 >30 min) that allow selective disruption of non-specific (or non-cognate) binding interactions by using a large excess of a polyanionic competitor.
Ligand exchange was accelerated by using a large excess of the ligands.
Further experiments need to be carried out to avoid using a large excess of pyruvate.
Its formation can be avoided by using a large excess of CuI in the reaction with 1.
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Because combustion turbines typically use a large excess of air, the resulting turbine exhaust gas is relatively dilute (only 3 4% CO2), making subsequent CO2 capture difficult and costly.
We use a large excess of adapters to drive ligation of every possible template strand.
However, we used a large excess of substrate (100 μg luciferin vs < 100 ng luciferase in the muscle i.e at minimum 1000 times more substrate), and can assume that diffusion will have not a great effect.
Our results suggest that the cross-linking chemistry may be more efficient under cellular conditions than might be predicted based on model systems that use a large excess of the electrophile.
After the macaroni has cooked, pour out the excess water, using a large spoon to prevent the noodles from falling into the sink or use a colander to drain then return noodles to pan.
More suggestions(15)
using a large photograph
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using a large shoot
using a national excess
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using a large water
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using a twofold excess
using a large capacitor
using a large reduction
using a large spoon
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