Briquettes wereproduced using a laboratory hydraulic press.
A central composite design (CCD) was used to determine the maximum gellan production at optimum values for three process parameters (Triton X-100 concentration, pH, and temperature) each at five levels in a laboratory fermentor.
To study animals, we can use a laboratory.
Figure 5 LBDW-fed cultivation of P. antarctica GB-4 0) GB-4 0a jar fermentor.
When kLa was selected as an index for scale-up and industrial scale production using a 15 m3 fermentor with the FULLZONE™ impeller was conducted, FR901379 production was successfully obtained at more than 50 U/mL, almost the same level as with the 0.03 m3 and 4 m3 fermentors.
For scale-up production of PaE, under xylose feeding cultivation of P. antarctica using a jar fermentor (Watanabe et al. 2014b), we found a highly secreted 33-kDa unknown protein (unpublished data).
These plasmids and the constitutive expression plasmid harbouring phaCABRe genes (pSYL105) were individually introduced into E. coli XL1-Blue, and a comparative study for P 3HB) production from glucose using a 10-l fermentor was carried out.
For the large-scale production of DR-ultrafiltrate used in radioprotection studies of E. coli and Jurkat T cells (Figure 1D, 1E and S1), D. radiodurans (ATCC BAA-816) was grown under optimized conditions using a 20-L fermentor [56].
For large scale purification, fermentation was performed using a 5 L fermentor as previously described [ 26].
More detailed analysis and fine-tuning of the aeration using a jar-fermentor is needed to optimize the culture conditions.
