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This study demonstrates that endonuclease mutants with altered specificity and non-lethal activity can be evolved towards more active variants using a laboratory evolution strategy.
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Briquettes wereproduced using a laboratory hydraulic press.
HS-AFM experiments were performed using a laboratory-built instrument57.
Here, we used an adaptive laboratory evolution method called visualizing evolution in real time (VERT) to help map out part of the adaptive landscape of Escherichia coli tolerance to the biofuel n-butanol.
Our strategy used a combination of two complementary genome-scale approaches: a screening of the haploid non-essential gene knockout collection [ 33, 34] and a laboratory evolution approach followed by whole genome resequencing [ 35].
This prediction has been supported by a laboratory evolution experiment with fruit flies [ 14].
To study animals, we can use a laboratory.
A prior study using adaptive laboratory evolution to increase thermotolerance of S. cerevisiae took over 300 generatoons20 to achieve this phenotype.
Here, E. coli lacking L-serine degradation pathways was evolved for improved tolerance by gradually increasing L-serine concentration from 3 to 100 g/L using adaptive laboratory evolution (ALE).
Dr. Ostermeier's lab seeks insight into the principles of natural evolution and the ability to design novel proteins and cells using laboratory evolution.
Using laboratory evolution, larger numbers of nondisruptive backbone fragmentation sites have been discovered within Thermotoga neapolitana adenylate kinase, E. coli methionyl tRNA synthetases, and T7 phage RNA polymerase.
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