Sentence examples for using a hypotonic solution from inspiring English sources
[GPP] A maximum fluid deficit of 1000 ml should be set when using a hypotonic solution in a healthy woman and surgery immediately stopped on reaching this limit.
Red blood cells were lysed using a hypotonic solution of ammonium chloride.
Cells were lysed using a hypotonic solution (10 mM HEPES pH 7.9, 10 mM KCl, 1.2 mM MgCl2, 0.3% NP-40 and 0.5 mM dithiothreitol) and dounce homogenized with a tight pestle.
Splenocytes were mechanically dissociated by disruption through steel mesh, and red blood cells were lysed using a hypotonic ammonium chloride solution (82.9 g ammonium chloride, 10 g potassium bicarbonate, 0.37 g EDTA, 1 l sterile water for a 10× solution).
Spleens of infected and uninfected mice were isolated by physical disruption and passage through 70 μm nylon mesh (BD Falcon), washed and erythrocytes lysed using a hypotonic Tris-buffered NH4Cl solution.
Red blood cells were lysed for 90 seconds using 24 mL of a hypotonic solution (10 mM Na2HPO4, 2 mM NaH2PO4; pH 7.2) followed by restoration using 12 mL of a hypertonic solution (10 mM Na2HPO4, 2 mM NaH2PO4, 461 mM NaCl; pH 7.2).
Using an appropriate hypotonic solution is the other critical element that has been emphasized in the current study.
For cytogenetic preparations, the cells were collected using trypsin and treated with an hypotonic solution: KCL (2.8 g/l), foetal calf serum 1/12 and they were fixed with 3 1 methanol/glacial acetic acid.
After growth for 3 days, adults from all plates are mixed and killed using a hypochlorite solution and embryos harvested in an hypotonic solution without food.
A hypotonic solution was made using PBS solution and distilled water; 2.5 ml of the PBS solution and 2.5 ml of distilled water were mixed in a cuvette.
Measurements of ploidy (DNA index, DI) was carried out by staining samples with a hypotonic solution of PI using an automated DNA staining device (DNA-prep reagents; Coulter) and analysed by flow cytometry (Epics XL; Coulter).
