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The amount of collagen synthesis was determined using a hydroxyproline assay.
Furthermore, the amount of collagen synthesis was determined using a hydroxyproline assay, and the presence of collagen types I and II was investigated using Western blotting.
Hydroxyproline content in cell culture media was measured using a hydroxyproline assay kit (Sigma-Aldrich).
Collagen was quantified in the pellets of the NP and AF by using a hydroxyproline assay [ 32].
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Hepatic hydroxyproline content was measured using a hydroxyproline detection kit (Jiancheng Institute of Biotechnology, Nanjing, China) according to the manufacturer's instructions.
Collagen levels were determined in lung homogenates using an established hydroxyproline assay as previously described [12].
To examine the amount of collagen fibres, a hydroxyproline assay was used to quantify the collagen content in eviscerated embryos at E14.5.
Proliferation was assessed with a DNA quantitation technique, collagen synthesis was measured via a hydroxyproline assay, and GAG synthesis was determined with a dimethylmethylene blue dye binding assay at 14 days.
Collagen synthesis is determined by a hydroxyproline assay [ 20].
The sample was hydrolyzed and hydroxyproline was quantified by a hydroxyproline assay kit (Nanjing Jiancheng Bioengineering Institute, CN).
To confirm that the observed increase in dermal thickness in bleomycin-treated skin was due to increased collagen content, type I fibrillary collagen was imaged by SHG (Fig. 1c) and a hydroxyproline assay (Fig. 1d) was performed.
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