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Analytes were detected using a hybrid quadrupole linear ion trap mass spectrometer that was equipped with an electrospray ionisation source in the negative ion and multiple-reaction monitoring modes.
Gradient-eluted peptides were ionized using an electrospray ionization source, modified for nanospray, and were analysed using a hybrid quadrupole linear ion trap Fourier transform (LTQ-FT) mass spectrometer (Thermo Scientific, MA, USA).
The Electrospray ionization (ESI-MS) mass spectra were acquired using a hybrid quadrupole orthogonal acceleration tandem mass spectrometer QTOF from Micromass Manchesterr, UK) fitted with a Z-spray nanoflow electrospray ion source.
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A targeted detection methodology, denoted as MS Poisson detection, acquires and analyzes MS spectra using a hybrid quadrupole-LIT instrument and a probabilistic measure.
The nanospray MS Poisson detection method characterized here measures and analyzes MS spectra of a complex sample using a hybrid quadrupole-LIT instrument.
Extracted metabolites were analyzed by LC-MS/MS using a hybrid linear ion trap triple quadrupole mass spectrometer under full scan, enhanced product ion scan, neutral loss scan and precursor scan modes.
25 The digested protein samples were then subjected to Q-TOF mass spectrometry using a hybrid quadrupole/orthogonal time-of-flight mass spectrometer, Q-TOF API US (Waters), interfaced with a capillary liquid chromatography system (Waters), which constitutes a complete liquid chromatography-tandem mass spectrometry (LC MS/MS) analysis.
The fractions were then analyzed using a hybrid Quadrupole/Time-of-flight MS (Triple-TOF 5600, AB SCIEX, USA) with nano electrospray ion source.
Peptides were desalted using C18 reversed-phase extraction using Pierce C-18 spin columns (Thermo) and analyzed by microcapillary LC-MS/MS using a hybrid quadrupole/atmospheric pressure ionization orthogonal accelerated time-of-flight mass spectrometer (MSI-Q-TOF; Bruker Daltonics).
To this end, we performed shotgun lipidomic analysis using MS/MSALL on a hybrid quadrupole TOF mass spectrometer [ 24].
The MS molecular detection experiments described in this study used a hybrid geometry in which a quadrupole mass filter transmits the parent ions, which are subsequently fragmented in a collision cell, and the fragments were collected in a downstream LIT (QTrap 4000).
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