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To investigate if the fluorescent-labeled NC and LNC could be observed by fluorescence microscopy, the nanoparticle uptake was evaluated using a human macrophage cell line.
Although an up-regulation of PECAM-1-gene-expression by TGF-β-treatment has been observed using a human macrophage cell line (U937 cells) [ 30], this is the first report showing similar effects on endothelial cells and on tissue macrophages.
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A human macrophage cell line was used as the cell model to evaluate the fluorescent nanoparticle uptake.
A human macrophage cell line was maintained in RPMI-1640-FBS-5% and used as source of xenogeneic macrophages for vaccine preparation.
To assess alcohol's effects on the inflammatory responses of macrophages, researchers have used a human monoblastic cell line, MonoMac6, which has many features of mature macrophages and has been used to model Kupffer cell responses (Zhang et al. 2001).
Never use a human toothpaste.
Autoantibodies from 245 patients were analyzed from January 2005 to March 2012 by the autoimmune target (AIT) test, which was developed to overcome the limitations of the antinuclear antibody test using the human macrophage cell line (IT-1) as a substrate [ 2].
This anti-inflammatory effect could be reproduced in vitro using isolated human macrophage cultures; the release of TNF, interleukin (IL -1β, IL -1βnd IL-68 in response to endotoxin wandsignificantly reduced by acetylcholine (ACh) and nicotine.
We describe a quantitative histological study of 34 breast biopsies using a marker for human macrophages, the monoclonal antibody EBM/11.
For the experiments with macrophages, primary human monocytes were differentiated into human monocyte derived macrophages (hMDM) using human macrophage colony-stimulating factor (hM-CSF).
Using human macrophage (MONO-MAC-6) cells, we determined the optimal filter numbers and passage frequencies for the LeukoCatch system.
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