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Insulin was determined using a human insulin ELISA kit (Alpco, Windham, NH).
Insulin radioimmunoassay (RIA) was performed to determine the levels of murine insulin I and II using a human insulin radioimmunoassay kit according to the manufacturer's instructions (MP Biomedicals, Irvine, CA).
Media and islets were recovered and insulin concentration was determined using a human insulin ELISA.
Plasma insulin was assessed by RIA using a human insulin standard (Novo Biolabs no 471), plasma glucagon was measured by competitive RIA using a reagent kit from Eurodiagnostica (Malmö, Sweden) and plasma cortisol was measured by Luminescence Immunoassay on an Architect random access analyser (Abbott, Hoofddorp, the Netherlands) [ 31].
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Processed human insulin was measured in the extracts using a human insulin-specific radioimmunoassay.
Serum insulin was measured using a human insulin-specific radioimmunoassay (Linco Research, St. Charles, MO).
Fasting serum insulin levels were determined using a human insulin-specific radioimmunoassay kit (Linco Research, St. Charles, MO).
Plasma insulin was measured using a human insulin-specific (no cross-reactivity with proinsulin) ELISA (Dako Cytomation, Ely, UK) and glucose by the glucose oxidase method.
If all dispensations for analogue insulin between 2000 and 2009 had used a human insulin alternative, the NHS would have saved an estimated £625 million.
In order to further explore the mechanism by which chromium picolinate induces insulin resistance, we used a human insulin signaling pathway PCR array to examine the expression of genes in skeletal muscle before and after chromium therapy.
Serum insulin concentrations were determined using a human insulin specific radioimmunoassay kit (Linco Research).
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