Instead of using a human punching bag".
Detection was carried out using an anti-human cyclin B1 (sc-752) (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) primary antibody.
To obtain cyclin E-overexpressing derivatives of Rat-1 fibroblasts, cells were transduced with a human cyclin E cDNA expressed from a retroviral promoter, as previously described.
Never use a human toothpaste.
Alternatively, you can use a human name.
Cyclin D1 expression was measured by intracellular protein staining using mAb to human cyclin D1 (clone 5D4) and Cytofix/CytoPerm kit (BD Biosciences), according to the instructions of the manufacturer.
In hamster cell, using human cyclin D1 promoter, data showed that DNA methylation was found at Sp1/CRE binding sites (Hilton et al. 2005).
Using human cyclin B, cyclin C, and cyclin Y proteins as queries, similar Blast searches were carried out to identify cyclin proteins from related organisms in the NCBI and Broad Institute databases.
Detection was carried out using anti-human cyclin B1 (ref. H433; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), phospho-Bcl2 (ref. 2827S; Cell Signaling Technology, Boston, MA, USA), caspase-3 (ref. 9665; Cell Signaling), GAPDH (ref. 2118; Cell Signaling) and p53 (ref. ab28-100; AbCambridgeridge, UK) primary antibodies.
The total cyclin D1 and cyclin E contents were quantitated using mouse anti-human cyclin D1 and mouse anti-human cyclin E antibodies, respectively (Cell Signaling Technology, Beverly, MA, USA), followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Sigma).
