Sentence examples for using a human cDNA from inspiring English sources

Here we analyzed signaling crosstalk-specific profiles with MCF7 cells using a human cDNA array.

Amplification of SEC61B was carried out using a human cDNA bank as template.

Human Ciapin1 ORF was amplified using oligos 160 and 161 (see Table 2) using a human cDNA library made from Hela cells (kind gift from Olga Grigorieva) and subcloned into pRS315-Tef1 and pRS425-Tef1 promoter using SpeI-NotI.

We previously conducted a screen for genes aberrantly expressed in three ovarian adenocarcinoma cell lines compared to an immortalised HOSE using a human cDNA array containing 588 known genes (Arnold et al, 2001a).

HIPK4 was PCR amplified using a human cDNA clone (MGC 26964, Openbiosystems, Huntsville, AL) as template and ligated into pEGFP-C1 using N-terminal BglII and C-terminal EcoRI sites.

Vector expressing human neuroglobin was generated using a human cDNA clone from Origene (Rockville, MD, USA) subcloned into the KpnI and XbaI sites of pcDNA3.1 and the construct verified by direct sequencing.

Dazap1 cDNA clones were isolated from a mouse testis cDNA library (#937308, Stratagene, La Jolla, CA) using a human DAZAP1 cDNA as a probe.

A cDNA for human Abi-2 was amplified by means of a PCR using a human leukocyte cDNA library (CLONTECH).

In this study we performed a yeast two-hybrid screening using a human colon cDNA library and found that Grb2 and GrpL are binding partners of HD-PTP.

The CRIB domain of PAK1 (a.a. 67 150) was amplified by means of a PCR using a human leukocyte cDNA library as a template.

Cultured keratinocytes were stimulated with IL-21 and assessed for differential expression compared with nonstimulated controls using a human Atlas cDNA expression array (Clontech, Palo Alto, CA, USA).