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In the HPDC set of casting trials, melt velocities were increased by using a higher plunger velocity.
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Use a high setting.
San Fransisco resident Bhautik Joshi is on the second build of his custom made tilt-shift lens, the first one was built using a toilet plunger of all things.
The cell suspension was filtered through a 70 μm-filter using a syringe plunger, washed in PBS, and centrifuged.
We retrieved NSHDS samples from their cryovials in a frozen state by making a small opening at the bottom of each vial using a hot plunger and pushing the sample out with another plunger.
Single cell suspensions were prepared by forcing nodes through a 70 μm cell strainer using a syringe plunger.
Single-cell suspensions were prepared by forcing tissue through a fine wire mesh using a syringe plunger followed by repeated pipetting in culture medium.
Digested material was passed through a 70 μm cell strainer (Falcon, BD Franklin Lakes USA), remaining tissue pieces were minced through the strainer using a syringe plunger.
T. orientalis extract or vehicle was applied topically on the dorsal skin for 21 days using a syringe plunger with the same strokes.
The anesthetized embryos were pulled into the small glass capillaries containing 1% low-melting agarose in E3 medium, using a metal plunger.
Both organs were homogenised separately by gently pressing them through the 70- μm cell strainer, using a syringe plunger to make single-cell suspensions.
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